Win/MacRhizo, an advanced analysis system for the scientific study of roots
 For Root Morphology
And Architecture Measurement


Measurements
WinRHIZO (for washed root measurement) is available in three versions:
Basic, Regular & Pro
  • WinRHIZO Basic is a low cost entry level version. It produces only global measurements (average root diameter, total root length, area, volume and number of tips).
  • WinRHIZO Regular has all features of the basic model plus root morphology measurement as a function of user definable diameter classes. A root (length, area, volume) distribution graphic is automatically generated and is visible above the image. This graphic can also be printed.
  • WinRHIZO Pro has all features of the regular version plus link, topology, architecture and color analyses. The color analysis can produce root morphology as a function of color. It can also be used as a color area meter.

    Note: It is possible, at any time, to upgrade from a lower to a higher program version by paying the difference in cost between the two versions at the time of upgrade.
Measurements in function of WinRHIZO versions
Measurement
Model
Global (total of the whole image)
BASIC
REG
PRO
Total Length
Yes
 Yes Yes
Average Diameter
Yes
 Yes Yes
Total Area
Yes
 Yes Yes
Total Volume
Yes
Yes
Yes
Number of tips, forks & crossings
Yes
 Yes Yes
In function of root diameter (per diameter class)
Length
No
 Yes Yes
Area
No
 Yes Yes
Volume
No
 Yes Yes
Number of tips
No
 Yes Yes
In function of root color (see Color Analysis)
Length
No
No
 Yes
Area
No
No
 Yes
Volume
No
No
 Yes
Number of tips
No
No
 Yes
Link Analysis
Link analysis is a study of the morphology and basic connectivity of root segments. It can be done on incomplete or complete root systems.
BASIC REG PRO
Globally (for the whole image)
No
No
Yes
Total number of links
No
No
Yes
Average link length
No
No
Yes
Average link area
No
No
Yes
Average link diameter
No
No
Yes
Average branching angle
No
No
Yes
Per link (individually)
Length
No
No
Yes
Average diameter
No
No
Yes
Area
No
No
Yes
Branching angle
No
No
Yes
Internal-External classification
No
No
Yes
WinRHIZO link analysis
  • Link analysis: Segment length, average diameter, surface area, volume, branching angle and connectivity (external-internal).
  • Topology: Link developmental order classification (primary, secondary, tertiary...) with different order continuation criteria (smallest branching angle, largest diameter or highest magnitude) on simple and spread root systems. Also included are Link classification (external, internal), Path length, Altitude, Magnitude.
  • Root architecture with fractals.
  • Color analysis which allows you to do the multiple measurements.
Branching angle
WinRHIZO internal external classification
Internal-External classification
Red = External-External link
Yellow = External-Internal link
Green = Internal-Internal link
Blue = Isolated link (not connected to any link
Topology

Topology analysis is an extensive link connectivity analysis.

  • Magnitude, the number of external links extending from a link.
  • Path length, the number of links between a link and the base link, (inclusively).
  • External path length, the sum of path lengths of all external links. It is a value for the complete root system (not per link).
  • Altitude, the largest path length.
WinRHIZO topology
 WinRHIZO topology
  • Connectivity gives the links number that a link is connected to including;
    1) Descending links (children), 2) Same level links (siblings) and 3) Ascending links (parents)
BASIC
REG
PRO
Globally (for the whole image)
No
No
Yes
External path length
No
No
Yes
Altitude
No
No
Yes
Per link (individually)
Magnitude
No
No
Yes
Path length
No
No
Yes
Altitude
No
No
Yes
Connectivity
No
No
Yes
Note: Topology and developmental analyses require an integral root system. It is meaningless if the connectivity of the root system’s links has been destroyed by manipulation or the imaging process. It is not recommended to do link analyses on dense root systems. If you cannot visually track root segments in an image, it is unlikely that WinRHIZO will be able to do so either. Measurements made on such images will not be precise and might contain errors.
Development classification, Fractals and Color Analysis
The developmental analysis tries to recreate the order in which links were created from the base link as the plant grew.

Links of the same order can also be grouped per axis. An axis is a group of connected links of the same order. Morphological data can be obtained for all orders and axes.
The axis of order 0 is shown in green. It is made of links #0, 1, 9, 10, 11, 17.WinRHIZO development classification, fractals and color analysis
There are 5 axis of order 1 (shown in blue):
One made of links #2, 3, 7
one made of links #12, 16
one made of link #18
one made of link #19
one made of links #20, 21
Developmental classification
BASIC
REG
PRO

Number of links (per order)
No
No
Yes

Total length (per order)
No
No
Yes

Total area (per order)
No
No
Yes

Average link length (per order)
No
No
Yes

Average link area (per order)
No
No
Yes

Average diameter (per order)
No
No
Yes

Link order (per link)
No
No
Yes
Root Architecture with Fractals
No
No
Yes
Color Analysis (click here)
No
No
Yes

To learn more about WinRHIZO click the following links or you can download our WinRHIZO brochure (1.3 MB).

WinRHIZO.

WinRHIZO program for washed root analysis is available in three versions.

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WinRHIZO image acquisition components.
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WinRHIZO and MacRHIZO are registered trademarks
Copyright (c) 1997-2008 by Regent Instruments Inc. for entire contents
All rights reserved.
www.regentinstruments.com

Last Revision: January 23, 2008